
The Nano-Check™ Nicotine test is a rapid, self-controlled immunoassay for the qualitative detection of cotinine in human urine. Cotinine is a primary metabolite of nicotine and remains in the body of habitual tobacco users for approximately 17 hours. The cutoff concentration of this test is 200 & 500 ng/ml of cotinine. This in-vitro assay is intended for professional use.
:: Allow test device and the urine specimen to reach room temperature (15-30°C) before testing.
1. Bring the packet to room temperature before using it. Remove the nicotine test device from the sealed packet and use it as soon as possible.
2. Place the nicotine (cotinine) test device on a clean surface. Hold the dropper vertically and transfer 3 drops of urine (approx. 100 µL) to the specimen well (S) of the test device, and then start the timer. Avoid trapping air bubbles in the specimen well (S).
3. Wait for the colored line(s) to appear. The result should be read at 5 minutes. It is vital that the background is clear before reading the result. The results should not be interpreted after 10 minutes.

:: Interpretation of Results
NEGATIVE:* Two lines appear. One colored line should be in the control line region (C), and another colored line should be in the test line region (T). This negative result indicates that the Cotinine (COT) concentration is below the detectable level (200 ng/mL).
*NOTE: The shade of color in the test line region (T) may vary, but it should be considered negative whenever there is even a faint line.
POSITIVE: One colored line appears in the control line region (C). No line appears in the test line region (T). This positive result indicates that the Cotinine (COT) concentration exceeds the detectable level (200 ng/mL).
INVALID: Control line fails to appear. Insufficient specimen volume or incorrect procedural techniques are the most likely reasons for control line failure. Review the procedure and repeat the test using a new test device.
This Cotinine (COT) Nicotine rapid test is used for detection of cotinine in human urine. It is used for various purposes like pre insurance policy health check up, employment screening, clinical trials etc. Various pathological laboratories and Clinical Reasearch Organization (CRO) use this FDA approved rapid nicotine test kit for their medical research process. This nicotine urine test is very useful in cotinine detection of person under the influence of tobacco / nicotine by cigarette smoking, chewing tobacco, snuff tobacco, drugs etc.
Pammvi exclusively represents Nano-Ditech - USA for their rapid nicotine drug urine test kit in India. As a specialist Drug of Abuse test kit supplier, our cotinine (COT) nicotine urine rapid test kits have found acceptance with a wide spectrum of quality conscious institutional buyers. Our tobacco (nicotine) tests adhere to the most stringent quality certifications which are universally accepted.
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